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Horse Interleukin 1 Beta ELISA Kit 0.2ng/L - 70ng/L Standard Curve Range for Research Use

    Buy cheap Horse Interleukin 1 Beta ELISA Kit 0.2ng/L - 70ng/L Standard Curve Range for Research Use from wholesalers
     
    Buy cheap Horse Interleukin 1 Beta ELISA Kit 0.2ng/L - 70ng/L Standard Curve Range for Research Use from wholesalers
    • Buy cheap Horse Interleukin 1 Beta ELISA Kit 0.2ng/L - 70ng/L Standard Curve Range for Research Use from wholesalers

    Horse Interleukin 1 Beta ELISA Kit 0.2ng/L - 70ng/L Standard Curve Range for Research Use

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    Brand Name : BT Lab
    Model Number : Cat.No E0079Ho
    Certification : CE, ISO9001:2005, MSDS
    Price : Negotiation
    Payment Terms : Western Union, T/T
    Supply Ability : In Stock
    Delivery Time : 1-3 business days, bulk order within one week
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    Horse Interleukin 1 Beta ELISA Kit 0.2ng/L - 70ng/L Standard Curve Range for Research Use

    96 wells Specificity Horse Interleukin 1 Beta ELISA Kit


    Cat.No E0079Ho

    Standard Curve Range: 0.2ng/L - 70ng/L

    Sensitivity: 0.091ng/L

    Size: 96 wells

    Storage: Store the reagents at 2-8°C. For over 6-month storage refer to the expiration date keep it at -20°C. Avoid repeated thaw cycles. If individual reagents are opened it is recommended that the kit be used within 1 month.

    * This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.


    Intended Use

    This sandwich kit is for the accurate quantitative detection of Horse Interleukin 1 Beta (also known as IL-1B) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.


    Assay Principle

    This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Horse IL-1B antibody. IL-1B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Horse IL-1B Antibody is added and binds to IL-1B in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL-1B antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Horse IL-1B. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.


    Reagent Provided

    ComponentsQuantity
    Standard Solution (80ng/L)0.5ml x1
    Pre-coated ELISA Plate12 * 8 well strips x1
    Standard Diluent3ml x1
    Streptavidin-HRP6ml x1
    Stop Solution6ml x1
    Substrate Solution A6ml x1
    Substrate Solution B6ml x1
    Wash Buffer Concentrate (30x)20ml x1
    Biotinylated Horse IL-1 Beta Antibody1ml x1
    User Instruction1
    Plate Sealer2 pics
    Zipper bag1 pic

    Specimen Collection

    Serum Allow serum to clot for 10-20 minutes at room temperature. Centrifuge at 2000-3000 RPM for 20 minutes.


    Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C within 30 minutes of collection.


    Urine Collect by sterile tube. Centrifuge at 2000-3000 RPM for approximately 20 minutes. When collecting pleuroperitoneal fluid and cerebrospinal fluid, please follow the procedures above-mentioned.


    Cell Culture Supernatant Collect by sterile tubes when examining secrete components. Centrifuge at 2000-3000 RPM for approximately 20 minutes. Collect the supernatants carefully. When examining the components within the cell, use PBS (pH 7.2-7.4) to dilute cell suspension to the cell concentration of approximately 1 million/ml. Damage cells through repeated freeze-thaw cycles to let out the inside components. Centrifuge at 2000-3000 RPM for approximately 20 minutes.


    Tissue Rinse tissues in PBS (pH 7.4) to remove excess blood thoroughly and weigh before homogenization. Mince tissues and homogenize them in PBS (pH7.4) with a glass homogenizer on ice. Thaw at 2-8°C or freeze at -20°C. Centrifuge at 2000-3000 RPM for approximately 20 minutes.


    40ng/LStandard No.5120μl Original Standard + 120μl Standard Diluent
    20ng/LStandard No.4120μl Standard No.5 + 120μl Standard Diluent
    10ng/LStandard No.3120μl Standard No.4 + 120μl Standard Diluent
    5ng/LStandard No.2120μl Standard No.3 + 120μl Standard Diluent
    2.5ng/LStandard No.1120μl Standard No.2 + 120μl Standard Diluent

    Standard ConcentrationStandard No.5Standard No.4Standard No.3Standard No.2Standard No.1
    80ng/L40ng/L20ng/L10ng/L5ng/L2.5ng/L

    Wash Buffer Dilute 20ml of Wash Buffer Concentrate 30x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.


    Product Tags:

    elisa assay kit

      

    elisa reagent kit

      
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