Mouse CD 40L elisa kit

The kit is for the quantitative level of CD 40L in the sample, adopt purified Mouse CD 40L to coat microtiter plate, make solid-phase antibody, then add CD 40L to wells, Combine MCP-1 antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength