Mouse VEGF A elisa kit

The kit is for the quantitative level of VEGF A in the sample, adopt purified Mouse VEGF A to coat microtiter plate, make solid-phase antibody, then add VEGF A to wells, Combine IL6 antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of