Rat prostate specific antigen,PSA ELISA Kit

The kit is for the quantitative level of PSA in the sample, adopt purified Rat PSA to coat microtiter plate, make solid-phase antibody, then add PSA to wells, Combine PSA antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm.